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Журнал высшей нервной деятельности им. И.П. Павлова, 2022, T. 72, № 6, стр. 851-861

Вклад трансглутаминазы в индукцию и поддержание долговременной синаптической потенциации в нейронах виноградной улитки

А. Б. Зюзина 1, *, П. М. Балабан 1

1 Федеральное государственное бюджетное учреждение науки Институт высшей нервной деятельности и нейрофизиологии РАН
Москва, Россия

* E-mail: lucky-a89@mail.ru

Поступила в редакцию 11.03.2022
После доработки 31.03.2022
Принята к публикации 26.04.2022

Аннотация

Ранее было показано, что для успешного формирования долговременной потенциации у наземной улитки Helix lucorum необходим нейромедиатор серотонин. В последнее время в литературе накапливается все больше данных о важной роли серотонина не только как агента, действующего через синаптические рецепторы, но также посредством ковалентного присоединения к своим белковым мишеням внутри клетки путем серотонилирования. Ферментом, обеспечивающим данную модификацию, является трансглутаминаза типа II (трансглутаминаза). В целом на сегодняшний день не сообщалось об исследованиях, направленных на выяснение роли трансглутаминаз в серотонин-зависимой пластичности. В текущем исследовании мы впервые изучили влияние блокады трансглутаминазы с помощью ингибитора монодансилкадаверина на формирование долговременной потенциации синаптических ответов в идентифицированных премоторных (командных) нейронах оборонительного поведения виноградной улитки. Мы показали, что применение ингибитора трансглутаминазы монодансилкадаверина нарушает позднюю фазу долговременной потенциации амплитуды синаптического ответа, вызванной пятикратной тетанизацией сенсорного нерва (второго кожного или интестинального), совмещенной с аппликацией серотонина на in vitro-препарате изолированной центральной нервной системы. Мы также обнаружили, что аппликация монодансилкадаверина сама по себе не влияет на синаптическую передачу в премоторных нейронах. Полученные результаты позволяют предположить, что для поддержания индуцированной серотонином поздней фазы долговременной потенциации синаптических ответов в премоторных (командных) нейронах оборонительного поведения виноградной улитки требуется опосредованное трансглутаминазой серотонилирование.

Ключевые слова: серотонин, синаптическая пластичность, долговременная потенциация, трансглутаминаза, монодансилкадаверин, виноградная улитка

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